One of the new additions made to the 2020 remake of Trials of Mana is that of Li'l Cactus, a recurring character in more recent Mana series titles. While he was not in the original Seiken Densetsu 3 for Super Famicom, in the modern remake, he exists as a sort of collectible, appearing in 50 distinct places throughout the game world.
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It is very much worth keeping an eye out for Li'l Cactus, as he can offer some tremendous bonuses to your game. Not only can he give a discount at shops to start, but Li'l Cactus can also show treasure chests on your map, give random 2x and 3x boosts to your EXP after a battle, and ultimately an ability that will give your entire party 200% CS at the start of every single battle - definitely worth it.
This little side quest begins when you head to Golden Road for the first time. Li'l Cactus' first sighting is required, and cannot be missed. While he can show up in the areas of the game you play in before that point, which varies by character, he won't actually show up until after this first meeting at Golden Road, so you might have to re-explore some earlier areas a second time to seek him out.
Trials of Mana Cactus guide: how to find all the Lil Cactus
It is important to note, none of Li'l Cactus's locations are missable. While Trials of Mana does have a few one-time-only areas in the game that you cannot revisit, such as the Ghost Ship or Beuca Island, Li'l Cactus will never show up in these areas, so don't fret. Even if you get to right before the final boss of the game, you can still go back and seek out Li'l Cactus if you need to. If you decide to go into New Game Plus, all your Li'l Cactus sightings and rewards will also be carried over.
One final note: Trials of Mana has three different final dungeons depending on which character is your main, first-picked one. Duran & Angela go to the Crystal Desert, Charlotte & Kevin head through the Jungle of Visions, while Riesz & Hawkeye go to the Night Cavern. Two of Li'l Cactus' locations are in this penultimate dungeon, so that means #s 48 and 49 below are listed three times each, once for each dungeon.
The list of Li'l Cactus locations can be seen below, listed in order generally how you would find them throughout the game, though the later sections of the game can be tackled in various order.
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Trials of Mana - Li'l Cactus Locations
1 - Golden Road - right on the path, can't miss.
2 - Free City Maia - on an awning near the boat.
3 - Dwarf Tunnel I - By a campfire
4 - Molebear Moors I - On a ledge near the entrance to Stonesplit Gap.
5 - Stonesplit Gap II - On the Valsena side of the broken bridge.
6 - Valsena, Kingdom of the Plains - NE corner after you repel Altenish forces from Valsena.
7 - Merchant Town Beiser - West of the weapon shop.
8 - Palo Port - West of the armor shop.
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9 - Heavensway I - At beach in close to Palo Port.
10 - Gusthall I - Alcove to the left as you enter from Heavensway.
11 - Heavensway I - Near the waterfall past the somnosa flower field near the Laurent Secret Base.
12 - Jadd Stronghold I - Southwest section of Jadd.
13 - Rabite Forest (revisit) - Beach outside of Jadd.
14 - Lakesite Town Astoria (revisit) - Stop by Astoria on the way to Domperi.
15 - Rabite Forest (revisit) - Southernmost section, near the Koropokkur Woods.
16 - Gusthall I - Room on west side, use wind jump to lengthen your jump to him.
17 - Oblivisle - West side on the main path, near the trees.
18 - Snoy Hamlet Alrant - NE corner.
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19 - Frostbite Fields - North near exit to Alrant.
20 - Frostbite Fields - West of the gold statues, across the bridge.
21 - Labyrinth of Ice I - Right next to the Mana stone.
22 - Desert Capital Sirhtan - NE part of town.
23 - Burning Sands - Hiding among plants near the three Bulettes in the North section.
24 - Burning Sands - On an upper ledge in the center of Burning Sands.
25 - Oasis of Diin - North near the piers.
26 - Fiery Gorge I - In plain sight above the second lava pit.
27 - Moonlight Town Mintas - NW of Inn.
28 - Duskmoon Forest - Up a ledge where oyu climb a branch near the North.
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29 - Duskmoon Forest - S most area near the bridge.
30 - Chartmoon Tower - Right next to the Mana stone.
31 - Lampbloom Woods III - After the Red FLower maze.
32 - Flowerburg Dior - West side.
33 - Lampbloom Woods I - South of golden statue at northermost end of Woods.
34 - Laurent I - On the other side of the gate, gotten after all the elementals.
35 - Molebear Moors I - Left side you reach with Flammie.
36 - Labyrinth of Ice II - South of the gold statue, need to jump across ice platforms
37 - Fiery Gorge I - Near treasure chest in Benevedon section.
38 - Daria, Gem Valley I - On stairs near first treasure chest.
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39 - Daria, Gem Valley I - Near posion mist.
40 - Woods of Wandara III - In a large stump.
41 - Woods of Wandara VII - Below ramp.
42 - Holy City Wendel - NE side.
43 - Cascade Cavern I - Near chest right of Golden Statue.
44 - Chartmoon Tower VIII - At the top of the tower, NW corner.
45 - Shimmering Ruins - West side.
46 - Shimmering Ruins - Southern most room.
47 - Ancient Capital Pedda - SE corner.
48a (Duran or Angela) - Crystal Desert II - In the cave.
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48b (Kevin or Charlotte) - Jungle of Visions II - Near the middle gray statue.
48c (Riesz or Hawkeye) - Night Cavern IV - East side.
49a (Duran or Angela) - Crystal Desert I - Below the middle chest.
49b (Kevin or Charlotte)- Jungle of Visions IV - Near gold statue.
49c (Riesz or Hawkeye) - Night Cavern VI - Ledge near the entrance.